验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Calu-1 cells, HeLa cells, MDA-MB-468 cells, SH-SY5Y cells, SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27573-1-AP targets MAP7D3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26082 Product name: Recombinant human MAP7D3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 301-400 aa of NM_001173516 Sequence: ASVDAPPQVNVEVFCNTSMEASPKAGVGMAPEVSTDSFPVVSVDVSPVVSTYDSEMSMDASPELSIEALPKVDLETVPKVSIVASPEASLEAPPEVSLEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MAP7 domain containing 3 |
| 别名 | MAP7 domain containing 3, MDP3 |
| 计算分子量 | 98 kDa |
| 观测分子量 | 125-130 kDa |
| GenBank蛋白编号 | NM_001173516 |
| 基因名称 | MAP7D3 |
| Gene ID (NCBI) | 79649 |
| RRID | AB_3742239 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWC1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP7D3, also known as MDP3, features two coiled-coil regions near its N terminus, followed by a linker region, a MAP7-like domain, and a C-terminal domain. MAP7D3 is a microtubule-binding protein that regulates microtubule assembly and stability. MAP7D3 expression is cell cycle dependent, with lower expression in the G2 phase compared to its expression in the other phases of the cell cycle(PMID: 22142902).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP7D3 antibody 27573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
