验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A431 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MAP7 Ready-To-Use IHC Kit. MAP7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13446-1-AP targets MAP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4276 Product name: Recombinant human MAP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 400-749 aa of BC025777 Sequence: GRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREWEEAERAQRQKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 7 |
| 别名 | Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115 |
| 计算分子量 | 749 aa, 84 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC025777 |
| 基因名称 | MAP7 |
| Gene ID (NCBI) | 9053 |
| RRID | AB_10644485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14244 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP7 antibody 13446-1-AP | Download protocol |
| IHC protocol for MAP7 antibody 13446-1-AP | Download protocol |
| IF protocol for MAP7 antibody 13446-1-AP | Download protocol |
| IP protocol for MAP7 antibody 13446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites. | ||
Adv Sci (Weinh) N6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation | ||
Front Pharmacol A telomere-related signature for predicting prognosis and assessing immune microenvironment in osteosarcoma |
