• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30716-1-AP

    WB analysis using 30716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30716-1-AP (MAP4K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 30716-1-AP

    IHC staining of mouse skeletal muscle using 30716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 30716-1-AP

    IHC staining of mouse skeletal muscle using 30716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-87 MG using 30716-1-AP

    IF Staining of U-87 MG using 30716-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAP4K3 antibody (30716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MAP4K3 Polyclonal antibody

MAP4K3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30716-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30716-1-AP targets MAP4K3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP4K3 fusion protein Ag33512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase kinase 3
别名 MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase
计算分子量 101 kDa
观测分子量 99-101 kDa
GenBank蛋白编号BC071579
基因名称 MAP4K3
Gene ID (NCBI) 8491
RRIDAB_3086398
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVH8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

实验方案

Product Specific Protocols
WB protocol for MAP4K3 antibody 30716-1-APDownload protocol
IHC protocol for MAP4K3 antibody 30716-1-APDownload protocol
IF protocol for MAP4K3 antibody 30716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols