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验证数据展示

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 16212-1-AP

    IF Staining of HeLa using 16212-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAP3K6 antibody (16212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MAP3K6 Polyclonal antibody

MAP3K6 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Mitogen-activated protein kinase kinase kinase 6, MEKK6, MAPKKK6, EC:2.7.11.25, ASK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16212-1-AP targets MAP3K6 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP3K6 fusion protein Ag9206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 6
别名 Mitogen-activated protein kinase kinase kinase 6, MEKK6, MAPKKK6, EC:2.7.11.25, ASK2
计算分子量1148aa,128 kDa; 1011aa,113 kDa; 797aa,89 kDa
观测分子量 88 kDa
GenBank蛋白编号BC015914
基因名称 MAP3K6
Gene ID (NCBI) 9064
RRIDAB_2878229
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95382
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MAP3K6 antibody 16212-1-APDownload protocol
IF protocol for MAP3K6 antibody 16212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols