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验证数据展示

  • WB analysis of human skeletal muscle using 19974-1-AP

    WB analysis of human skeletal muscle using 19974-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 19974-1-AP

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 19974-1-AP

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 40x lens).

MLK2 Polyclonal antibody

MLK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19974-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19974-1-AP targets MLK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 10
别名 MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST
计算分子量 104 kDa
观测分子量 104-115 kDa
GenBank蛋白编号NM_002446
基因名称 MAP3K10
Gene ID (NCBI) 4294
RRIDAB_10732824
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02779
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K10. The antibody has no cross reaction to MAP3K9.

实验方案

Product Specific Protocols
WB protocol for MLK2 antibody 19974-1-APDownload protocol
IHC protocol for MLK2 antibody 19974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Lab Chip

Paper-based microreactor array for rapid screening of cell signaling cascades.

Authors - Chia-Hao Huang
View Article
humanWB

J Biol Chem

Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division.

Authors - Luis Cedeno-Rosario
View Article