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MEK5 Polyclonal antibody

MEK5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15758-1-AP

Print datasheet

Synonyms

HsT17454, MAP kinase kinase 5, MAP2K5, MAPK/ERK kinase 5, MAPKK 5, MAPKK5, MEK 5, MEK5, MKK5, PRKMK5

验证数据展示

  • WB analysis of HEK-293T using 15758-1-AP

    WB analysis of HEK-293T using 15758-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15758-1-AP (MEK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15758-1-AP

    IF Staining of HeLa using 15758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK5 antibody (15758-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human heart using 15758-1-AP

    IHC staining of human heart using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 15758-1-AP

    IHC staining of human heart using 15758-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15758-1-AP (MEK5 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293T cells, A431 cells, HeLa cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15758-1-AP targets MEK5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen MEK5 fusion protein Ag8351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 5
Synonyms HsT17454, MAP kinase kinase 5, MAP2K5, MAPK/ERK kinase 5, MAPKK 5, MAPKK5, MEK 5, MEK5, MKK5, PRKMK5
Calculated Molecular Weight 448 aa, 50 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC008838
Gene Symbol MAP2K5
Gene ID (NCBI) 5607
RRIDAB_2141275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13163
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MEK5 antibody 15758-1-APDownload protocol
IHC protocol for MEK5 antibody 15758-1-APDownload protocol
IF protocol for MEK5 antibody 15758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Hyperthermia

Decrease in MAP3Ks expression enhances the cell death caused by hyperthermia.

Authors - Atsushi Enomoto
View Article
ratWB

Pharm Biol

Injection of YiQiFuMai powder protects against heart failure via inhibiting p38 and ERK1/2 MAPKs activation.

Authors - Yongwei Nie
View Article