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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

    WB analysis using 67333-1-Ig (same clone as 67333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    IHC staining of human breast cancer using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67333-1-Ig (same clone as 67333-1-PBS)

    IF Staining of HepG2 using 67333-1-Ig (same clone as 67333-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAP2K4 antibody (67333-1-Ig, Clone: 2E4C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67333-1-PBS in a different storage buffer formulation.

MAP2K4 Monoclonal antibody, PBS Only

MAP2K4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67333-1-PBS

产品说明书

CloneNo. 2E4C1

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67333-1-PBS targets MAP2K4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 MAP2K4 fusion protein Ag11348 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 4
别名 MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2
计算分子量 399 aa, 44 kDa
观测分子量44-50 kDa
GenBank蛋白编号BC036032
基因名称 MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2882591
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45985
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.