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验证数据展示

  • WB analysis using 67333-1-Ig

    WB analysis using 67333-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67333-1-Ig

    IF Staining of HepG2 using 67333-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAP2K4 antibody (67333-1-Ig, Clone: 2E4C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67333-1-Ig

产品说明书

CloneNo. 2E4C1
引用文献(2)

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67333-1-Ig targets MAP2K4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag11348

Product name: Recombinant human MAP2K4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 50-399 aa of BC036032

Sequence: NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 4
别名 MEK4, 2E4C1, c-Jun N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC:2.7.12.2
计算分子量 399 aa, 44 kDa
观测分子量44-50 kDa
GenBank蛋白编号BC036032
基因名称 MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2882591
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP45985
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for MAP2K4 antibody 67333-1-IgDownload protocol
IHC protocol for MAP2K4 antibody 67333-1-IgDownload protocol
WB protocol for MAP2K4 antibody 67333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PNAS Nexus

A potent tumor-selective ERK pathway inactivator with high therapeutic index.

Authors - Zehua Zuo
View Article
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian
View Article
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