验证数据展示
经过测试的应用
| Positive IF-P detected in | rat brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67423 targets MAP1B in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | MAP1B fusion protein Ag17709 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1B |
| 别名 | Microtubule-associated protein 1B, MAP1B heavy chain, MAP-1B, MAP1 light chain LC1, 1E6B9 |
| 计算分子量 | 2468 aa, 271 kDa |
| GenBank蛋白编号 | BC141853 |
| 基因名称 | MAP1B |
| Gene ID (NCBI) | 4131 |
| RRID | AB_3084346 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P46821 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MAP1B antibody CL488-67423 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |