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验证数据展示

  • WB analysis of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    WB analysis of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.

  • IP experiment of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    IP experiment of human placenta using 14049-1-AP (same clone as 14049-1-PBS)

    IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug. This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.

MAN1A2 Polyclonal antibody, PBS Only

MAN1A2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14049-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14049-1-PBS targets MAN1A2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5240

Product name: Recombinant human MAN1A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-641 aa of BC063300

Sequence: VQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 2
别名
计算分子量 641 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC063300
基因名称 MAN1A2
Gene ID (NCBI) 10905
RRIDAB_2266033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60476
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).

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