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MAN1A2 Polyclonal antibody

MAN1A2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14049-1-AP

Print datasheet

Synonyms

Alpha 1,2 mannosidase IB, MAN1A2, MAN1B

验证数据展示

  • WB analysis of human placenta using 14049-1-AP

    WB analysis of human placenta using 14049-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 14049-1-AP

    IHC staining of human placenta using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 14049-1-AP

    IHC staining of human placenta using 14049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of human placenta using 14049-1-AP

    IP experiment of human placenta using 14049-1-AP

    IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human heart tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14049-1-AP targets MAN1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MAN1A2 fusion protein Ag5240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosidase, alpha, class 1A, member 2
Synonyms Alpha 1,2 mannosidase IB, MAN1A2, MAN1B
Calculated Molecular Weight 641 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC063300
Gene Symbol MAN1A2
Gene ID (NCBI) 10905
RRIDAB_2266033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).

实验方案

Product Specific Protocols
WB protocol for MAN1A2 antibody 14049-1-APDownload protocol
IHC protocol for MAN1A2 antibody 14049-1-APDownload protocol
IP protocol for MAN1A2 antibody 14049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

World J Gastroenterol

Hepatitis B virus upregulates host expression of α-1,2-mannosidases via the PPARα pathway.

Authors - Song Hu
View Article