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验证数据展示

  • WB analysis using 86094-1-RR (same clone as 86094-1-PBS)

    WB analysis using 86094-1-RR (same clone as 86094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86094-1-RR (MAN1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02326-1

    Sandwich ELISA standard curve of MP02326-1

    Sandwich ELISA standard curve of MP02326-1, Human MAN1A1 Recombinant Matched Antibody Pair - PBS only. 86094-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20060. 86094-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP02326-1

    Sample test of MP02326-1

    Urine of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 0.7 ng/mL with a range of ND-2.2 ng/mL.

  • Sample test of MP02326-1

    Sample test of MP02326-1

    Serum of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 11.9 ng/mL with a range of 6.8-24.2 ng/mL.

  • IHC staining of human ovary cancer using 86094-1-RR (same clone as 86094-1-PBS)

    IHC staining of human ovary cancer using 86094-1-RR (same clone as 86094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86094-1-RR (MAN1A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86094-1-RR (same clone as 86094-1-PBS)

    IF Staining of HepG2 using 86094-1-RR (same clone as 86094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (86094-1-RR, Clone: 250444H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86094-1-RR

    Affinity and Kinetic Characterization of 86094-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86094-1-RR against Human MAN1A1 were performed. The affinity constant is 1.04 nM.

MAN1A1 Recombinant monoclonal antibody, PBS Only (Capture)

MAN1A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86094-1-PBS

产品说明书

CloneNo. 250444H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86094-1-PBS targets MAN1A1 as part of a matched antibody pair:

MP02326-1: 86094-1-PBS capture and 86094-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag20060

Product name: Recombinant human MAN1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-367 aa of BC065827

Sequence: VLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEEIEARKGQANCPGSSSSCEVEIQMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKRWMCNIPRAMEATI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 1
别名 Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
计算分子量 653 aa, 73 kDa
观测分子量 68 kDa
GenBank蛋白编号BC065827
基因名称 MAN1A1
Gene ID (NCBI) 4121
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP33908
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

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