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验证数据展示

  • WB analysis using 86094-1-RR

    WB analysis using 86094-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86094-1-RR (MAN1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86094-1-RR

    IF Staining of HepG2 using 86094-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (86094-1-RR, Clone: 250444H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86094-1-RR

    Affinity and Kinetic Characterization of 86094-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86094-1-RR against Human MAN1A1 were performed. The affinity constant is 1.04 nM.

MAN1A1 Recombinant antibody

MAN1A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86094-1-RR

产品说明书

CloneNo. 250444H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, HEK-293 cells, human urine
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86094-1-RR targets MAN1A1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MAN1A1 fusion protein Ag20060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mannosidase, alpha, class 1A, member 1
别名 Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
计算分子量 653 aa, 73 kDa
观测分子量 68 kDa
GenBank蛋白编号BC065827
基因名称 MAN1A1
Gene ID (NCBI) 4121
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP33908
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

实验方案

Product Specific Protocols
WB protocol for MAN1A1 antibody 86094-1-RRDownload protocol
IF protocol for MAN1A1 antibody 86094-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols