验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| CoIP | See 1 publications below |
产品信息
55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mastermind-like 1 (Drosophila) |
| 别名 | KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1 |
| 计算分子量 | 108 kDa |
| 观测分子量 | 108-120 kDa |
| GenBank蛋白编号 | NM_014757 |
| 基因名称 | MAML1 |
| Gene ID (NCBI) | 9794 |
| RRID | AB_11182286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92585 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAML1 antibody 55493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Identification of SCAMP2 as a regulator of NOTCH signaling in cisplatin resistance through a novel prognostic model for bladder cancer | ||
Int J Biol Sci Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling. | ||
Discov Oncol CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway |
