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验证数据展示

  • WB analysis using 84771-4-RR (same clone as 84771-4-PBS)

    WB analysis using 84771-4-RR (same clone as 84771-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84771-4-RR (MAGI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    IHC staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84771-4-RR (MAGI3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    IF Staining of mouse brain using 84771-4-RR (same clone as 84771-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGI3 antibody (84771-4-RR, Clone: 242212C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84771-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84771-4-RR

    Affinity and Kinetic Characterization of 84771-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84771-4-RR against Human MAGI3 were performed. The affinity constant is 0.752 nM.

MAGI3 Recombinant monoclonal antibody, PBS Only

MAGI3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84771-4-PBS

产品说明书

CloneNo. 242212C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

242212C9, KIAA1634, MAGI-3, Membrane-associated guanylate kinase inverted 3, Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84771-4-PBS targets MAGI3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18394

Product name: Recombinant human MAGI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-777 aa of BC130409

Sequence: LPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCID

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane associated guanylate kinase, WW and PDZ domain containing 3
别名 242212C9, KIAA1634, MAGI-3, Membrane-associated guanylate kinase inverted 3, Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
计算分子量 1481 aa, 163 kDa
观测分子量140 kDa
GenBank蛋白编号BC130409
基因名称 MAGI3
Gene ID (NCBI) 260425
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ5TCQ9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

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