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验证数据展示

  • WB analysis using 84771-4-RR

    WB analysis using 84771-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84771-4-RR (MAGI3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84771-4-RR

    IHC staining of mouse brain using 84771-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84771-4-RR (MAGI3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84771-4-RR

    IF Staining of mouse brain using 84771-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAGI3 antibody (84771-4-RR, Clone: 242212C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84771-4-RR

    Affinity and Kinetic Characterization of 84771-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84771-4-RR against Human MAGI3 were performed. The affinity constant is 0.752 nM.

MAGI3 Recombinant monoclonal antibody

MAGI3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84771-4-RR

产品说明书

CloneNo. 242212C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

242212C9, KIAA1634, MAGI-3, Membrane-associated guanylate kinase inverted 3, Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84771-4-RR targets MAGI3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18394

Product name: Recombinant human MAGI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-777 aa of BC130409

Sequence: LPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCID

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 membrane associated guanylate kinase, WW and PDZ domain containing 3
别名 242212C9, KIAA1634, MAGI-3, Membrane-associated guanylate kinase inverted 3, Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
计算分子量 1481 aa, 163 kDa
观测分子量140 kDa
GenBank蛋白编号BC130409
基因名称 MAGI3
Gene ID (NCBI) 260425
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ5TCQ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

实验方案

Product Specific Protocols
IF protocol for MAGI3 antibody 84771-4-RRDownload protocol
IHC protocol for MAGI3 antibody 84771-4-RRDownload protocol
WB protocol for MAGI3 antibody 84771-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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