验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse thymus tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
21491-1-AP targets MAGEC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag15819 Product name: Recombinant human MAGEC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-130 aa of BC103893 Sequence: MPLFPNLPRLSFEEDFQNPSVTEDLVDAQDSIDEEEEDASSTSSSSFHFLFPSSSSLSSSSPLSSPLPSTLILGVPEDEDMPAAGMPPLPQSPPEIPPQGPPKISPQGPPQSPPQSPLDSCSSPLLWTRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma antigen family C, 3 |
| 别名 | Melanoma-associated antigen C3, MAGE-C3 antigen, Hepatocellular carcinoma-associated antigen 2, HCA2, CT7.2 |
| 计算分子量 | 643 aa, 72 kDa |
| 观测分子量 | 70-72 kDa |
| GenBank蛋白编号 | BC103893 |
| 基因名称 | MAGEC3 |
| Gene ID (NCBI) | 139081 |
| RRID | AB_10734430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TD91 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
| IHC protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
| WB protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma. | ||
BMC Cancer Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells. |
