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验证数据展示

  • WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    WB analysis using 83480-5-RR (same clone as 83480-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

    IF Staining of A549 using 83480-5-RR (same clone as 83480-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

    FC experiment of HeLa using 83480-5-RR (same clone as 83480-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83480-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00467-3

    Cytometric bead array standard curve of MP00467-3

    Cytometric bead array standard curve of MP00467-3, MAGEA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83480-1-PBS. Detection antibody: 83480-5-PBS. Standard: Ag0265. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83480-5-RR

    Affinity and Kinetic Characterization of 83480-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

MAGEA3 Recombinant antibody, PBS Only (Detector)

MAGEA3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83480-5-PBS

产品说明书

CloneNo. 240435G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAGEA3 Ready-To-Use IHC Kit. MAGEA3 primary antibody included.

产品信息

83480-5-PBS targets MAGEA3 as part of a matched antibody pair:

MP00467-3: 83480-1-PBS capture and 83480-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MAGEA3 fusion protein Ag0265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 3
别名 HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D
计算分子量 314 aa, 35 kDa
观测分子量45 kDa
GenBank蛋白编号BC000340
基因名称 MAGEA3
Gene ID (NCBI) 4102
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43357
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.