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验证数据展示

  • WB analysis of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    WB analysis of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    WB analysis of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

    WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

    WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    IHC staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAG/Siglec-4a antibody (86073-3-RR, Clone: 250740E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAG/Siglec-4a antibody (86073-3-RR, Clone: 250740E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

Rat MAG/Siglec-4a Recombinant monoclonal antibody, PBS Only

MAG/Siglec-4a Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86073-3-PBS

产品说明书

CloneNo. 250740E3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

1B236, Brain neuron cytoplasmic protein 3, Mag, Myelin-associated glycoprotein, Sialic acid-binding Ig-like lectin 4a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86073-3-PBS targets MAG/Siglec-4a in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3251

Product name: Recombinant Rat MAG protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-516 aa of NM_017190.4

Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myelin-associated glycoprotein
别名 1B236, Brain neuron cytoplasmic protein 3, Mag, Myelin-associated glycoprotein, Sialic acid-binding Ig-like lectin 4a
计算分子量69 kDa
观测分子量70-100 kDa
GenBank蛋白编号NM_017190.4
基因名称 Mag
Gene ID (NCBI) 29409
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07722-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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