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验证数据展示

  • WB analysis using 33199-1-AP (same clone as 33199-1-PBS)

    WB analysis using 33199-1-AP (same clone as 33199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33199-1-AP (MAFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 33199-1-AP (same clone as 33199-1-PBS)

    IF Staining of HeLa using 33199-1-AP (same clone as 33199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAFK antibody (33199-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

MAFK Polyclonal antibody, PBS Only

MAFK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 33199-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33199-1-PBS targets MAFK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38230

Product name: Recombinant human MAFK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-156 aa of NM_002360.3

Sequence: MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVAPSKVATTSVITIVKSTELSSTSVPFSAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
别名 Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK
计算分子量18 kDa，156aa
观测分子量18 kDa
GenBank蛋白编号NM_002360.3
基因名称 MAFK
Gene ID (NCBI) 7975
RRIDAB_3742891
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60675
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. MAFF, MAFG, and MAFK are the three vertebrate sMafs (PMID: 27058431). They act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2, and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PMID: 8932385; 9150357). In cancer cell lines, MAFK promotes epithelial-to-mesenchymal transition (EMT) by downregulating E-cadherin expression through the targeting of transmembrane glycoprotein nonmetastatic B (GPNMB), a potent inducer of EMT (PMID: 28400538; 36061142).

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