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验证数据展示

  • WB analysis using 33199-1-AP

    WB analysis using 33199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33199-1-AP (MAFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 33199-1-AP

    IF Staining of HeLa using 33199-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAFK antibody (33199-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MAFK Polyclonal antibody

MAFK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33199-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Calu-3 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33199-1-AP targets MAFK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38230

Product name: Recombinant human MAFK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-156 aa of NM_002360.3

Sequence: MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVAPSKVATTSVITIVKSTELSSTSVPFSAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
别名 Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK
计算分子量18 kDa，156aa
观测分子量18 kDa
GenBank蛋白编号NM_002360.3
基因名称 MAFK
Gene ID (NCBI) 7975
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60675
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. MAFF, MAFG, and MAFK are the three vertebrate sMafs (PMID: 27058431). They act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2, and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PMID: 8932385; 9150357). In cancer cell lines, MAFK promotes epithelial-to-mesenchymal transition (EMT) by downregulating E-cadherin expression through the targeting of transmembrane glycoprotein nonmetastatic B (GPNMB), a potent inducer of EMT (PMID: 28400538; 36061142).

实验方案

Product Specific Protocols
IF protocol for MAFK antibody 33199-1-APDownload protocol
WB protocol for MAFK antibody 33199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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