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验证数据展示

  • WB analysis using 55013-1-AP (same clone as 55013-1-PBS)

    WB analysis using 55013-1-AP (same clone as 55013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55013-1-AP (c-MAF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 55013-1-AP (same clone as 55013-1-PBS)

    WB analysis of HepG2 using 55013-1-AP (same clone as 55013-1-PBS)

    WB result of c-MAF antibody (55013-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HepG2 cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 55013-1-AP (same clone as 55013-1-PBS)

    WB analysis of HeLa using 55013-1-AP (same clone as 55013-1-PBS)

    WB result of c-MAF antibody (55013-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAF transfected HeLa cells. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    IP experiment of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    IP result of anti-c-MAF (IP:55013-1-AP, 4ug; Detection:55013-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    IF Staining of A431 using 55013-1-AP (same clone as 55013-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 55013-1-AP (c-MAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55013-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

c-MAF Polyclonal antibody, PBS Only

c-MAF Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 55013-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

MAF, c MAF, cMAF, Proto oncogene c Maf, Proto-oncogene c-Maf

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55013-1-PBS targets c-MAF in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
别名 MAF, c MAF, cMAF, Proto oncogene c Maf, Proto-oncogene c-Maf
计算分子量 42 kDa
观测分子量 42-52 kDa
GenBank蛋白编号NM_005360
基因名称 MAF
Gene ID (NCBI) 4094
RRIDAB_10863127
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75444
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .

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