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验证数据展示

  • FC experiment of MG-63 using 98756-2-RR

    FC experiment of MG-63 using 98756-2-RR

    1x10^6 MG-63 cells were intracellularly stained with 0.25 ug Anti-Human M-CSF Rabbit RecAb (98756-2-RR, Clone: 252446E3) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human tonsillitis using 98756-2-RR

    IHC staining of human tonsillitis using 98756-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98756-2-RR (M-CSF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 98756-2-RR

    IHC staining of human tonsillitis using 98756-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98756-2-RR (M-CSF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human M-CSF Rabbit Recombinant Antibody

M-CSF Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98756-2-RR

产品说明书

CloneNo. 252446E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

CSF 1, CSF1, CSF-1, Lanimostim, M CSF

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMG-63 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98756-2-RR targets M-CSF in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human M-CSF protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 1 (macrophage)
别名 CSF 1, CSF1, CSF-1, Lanimostim, M CSF
基因名称 M-CSF
Gene ID (NCBI) 1435
RRIDAB_3746323
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09603
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance. (PMID: 29734839; 29679908; 28202039)

实验方案

Product Specific Protocols
FC protocol for M-CSF antibody 98756-2-RRDownload protocol
IHC protocol for M-CSF antibody 98756-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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