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验证数据展示

  • FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

    FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed. This data was developed using the same antibody clone with 65669-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

    FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) (red) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65669-1-PBS in a different storage buffer formulation.

Anti-Mouse Ly-6C (HK1.4) Rat IgG2a Recombinant Antibody, PBS Only

Ly-6C Uni-rAbTM Recombinant Antibody for FC
Cat No. 65669-1-PBS

产品说明书

CloneNo. HK1.4

宿主/亚型

Rat / IgG2a

种属反应性

mouse

应用

FC

别名

Ly6c, Ly 6C, Ly6c1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

65669-1-PBS targets Ly-6C in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 N/A 种属同源性预测
宿主/亚型 Rat / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus C1
别名 Ly6c, Ly 6C, Ly6c1
GenBank蛋白编号BC010764
基因名称 Ly-6C
Gene ID (NCBI) 17067
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).