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验证数据展示

  • IF Staining of U2OS using CL488-68281

    IF Staining of U2OS using CL488-68281

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 LZTFL1 antibody (CL488-68281, Clone: 2C1F6 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-68281

    FC experiment of Jurkat using CL488-68281

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LZTFL1 (CL488-68281, Clone:2C1F6) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68281

产品说明书

CloneNo. 2C1F6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

2C1F6, Leucine zipper transcription factor-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy LZTFL1 Ready-To-Use IHC Kit. LZTFL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68281-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68281 targets LZTFL1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag10733

Product name: Recombinant human LZTFL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-299 aa of BC025988

Sequence: MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEDTFTIDEVSEVLNGLQAVVHSEVESELINTAYTNVLLLRQLFAQAEKWYLKLQTDISELENQELLEQVAEFEKAEITSSNKKPILDVTKPKLAPLNEGGTAELLNEEILRLQEENEKLKSRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSNLENTVAALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVREQLHMAEKELEKKFQQTAAYRNMKEILTKKNDQIKDLRKRLAQYEPED

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine zipper transcription factor-like 1
别名 2C1F6, Leucine zipper transcription factor-like protein 1
计算分子量 299 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC025988
基因名称 LZTFL1
Gene ID (NCBI) 54585
RRIDAB_3084465
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQ48
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LZTFL1 antibody CL488-68281Download protocol
FC protocol for CL Plus 488 LZTFL1 antibody CL488-68281Download protocol
Standard Protocols
Click here to view our Standard Protocols