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CoraLite® Plus 488-conjugated LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C1F6

Cat No : CL488-68281

Print datasheet

Synonyms

FLJ36386, LZTFL1

验证数据展示

  • FC experiment of Jurkat using CL488-68281

    FC experiment of Jurkat using CL488-68281

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LZTFL1 (CL488-68281, Clone:2C1F6) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68281-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68281 targets LZTFL1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen LZTFL1 fusion protein Ag10733 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name leucine zipper transcription factor-like 1
Synonyms FLJ36386, LZTFL1
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025988
Gene Symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQ48
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 LZTFL1 antibody CL488-68281Download protocol
Standard Protocols
Click here to view our Standard Protocols