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验证数据展示

  • FC experiment of human PBMCs using 98678-1-RR

    FC experiment of human PBMCs using 98678-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human LY86 Rabbit RecAb (98678-1-RR, Clone:252168G12), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Lymphocytes were gated.

  • FC experiment of human PBMCs using 98678-1-RR

    FC experiment of human PBMCs using 98678-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human LY86 Rabbit RecAb (98678-1-RR, Clone:252168G12)(red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Lymphocytes were gated.

Anti-Human LY86 Rabbit Recombinant Antibody

LY86 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98678-1-RR

产品说明书

CloneNo. 252168G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98678-1-RR targets LY86 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg3108

Product name: recombinant human LY86 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-162 aa of NM_004271.4

Sequence: GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte antigen 86
别名 Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1
计算分子量18 kDa
GenBank蛋白编号NM_004271.4
基因名称 LY86
Gene ID (NCBI) 9450
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95711
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

LY86, also known as protein MD-1, is a secreted glycoprotein that is associated with RP105 (TLR family proteins) and plays a significant role in the B cell surface expression of RP105. The RP105/MD-1 complex is expressed on immune cells, including B cells, macrophages, and dendritic cells (PMID: 37861500). By forming the RP105-MD-1 complex, LY86 fine-tunes recognition of bacterial lipopolysaccharide (LPS) and subsequent cytokine production, acting as both a facilitator and a brake on innate immune responses. LY86 influences sterile inflammatory conditions such as atherosclerosis, obesity, insulin resistance and cardiac remodeling, and is being explored as a biomarker for unstable carotid plaques and other immune-mediated diseases (PMID: 39085350).

实验方案

Product Specific Protocols
FC protocol for LY86 antibody 98678-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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