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验证数据展示

  • WB analysis using 87173-1-RR (same clone as 87173-1-PBS)

    WB analysis using 87173-1-RR (same clone as 87173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87173-1-RR (LY86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87173-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02941-1

    Sandwich ELISA sample test of MP02941-1

    The mean LY86 concentration was determined to be 25.36 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load, 24.32 ng/mL in RAW264.7 cell extract based on a 1.3 mg/mL extract load and 45.34 ng/mL in mouse spleen tissue extract based on a 6.4 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02941-1

    Sandwich ELISA standard curve of MP02941-1

    Sandwich ELISA standard curve of MP02941-1, Human LY86 Recombinant Matched Antibody Pair - PBS only. 87173-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3108. 87173-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • IHC staining of human tonsillitis using 87173-1-RR (same clone as 87173-1-PBS)

    IHC staining of human tonsillitis using 87173-1-RR (same clone as 87173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87173-1-RR (LY86 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87173-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 87173-1-RR (same clone as 87173-1-PBS)

    IF Staining of THP-1 using 87173-1-RR (same clone as 87173-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LY86 antibody (87173-1-RR, Clone: 252168G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87173-1-PBS in a different storage buffer formulation.

LY86 Recombinant monoclonal antibody, PBS Only (Capture)

LY86 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 87173-1-PBS

产品说明书

CloneNo. 252168G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87173-1-PBS targets LY86 as part of a matched antibody pair:

MP02941-1: 87173-1-PBS capture and 87173-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3108

Product name: recombinant human LY86 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-162 aa of NM_004271.4

Sequence: GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte antigen 86
别名 Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1
计算分子量18 kDa
观测分子量20 kDa
GenBank蛋白编号NM_004271.4
基因名称 LY86
Gene ID (NCBI) 9450
RRIDAB_3745388
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95711
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LY86, also known as protein MD-1, is a secreted glycoprotein that is associated with RP105 (TLR family proteins) and plays a significant role in the B cell surface expression of RP105. The RP105/MD-1 complex is expressed on immune cells, including B cells, macrophages, and dendritic cells (PMID: 37861500). By forming the RP105-MD-1 complex, LY86 fine-tunes recognition of bacterial lipopolysaccharide (LPS) and subsequent cytokine production, acting as both a facilitator and a brake on innate immune responses. LY86 influences sterile inflammatory conditions such as atherosclerosis, obesity, insulin resistance and cardiac remodeling, and is being explored as a biomarker for unstable carotid plaques and other immune-mediated diseases (PMID: 39085350).

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