验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HL-60 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
25872-1-AP targets LY6G6F in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag22467 Product name: Recombinant human LY6G6F protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 18-238 aa of BC137212 Sequence: DNMQAIYVALGEAVELPCPSPPTLHGDEHLSWFCSPAAGSFTTLVAQVQVGRPAPDPGKPGRESRLRLLGNYSLWLEGSKEEDAGRYWCAVLGQHHNYQNWRVYDVLVLKGSQLSARAADGSPCNVLLCSVVPSRRMDSVTWQEGKGPVRGRVQSFWGSEAALLLVCPGEGLSEPRSRRPRIIRCLMTHNKGVSFSLAASIDASPALCAPSTGWDMPWILM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte antigen 6 complex, locus G6F |
| 别名 | C6orf21, G6f, LY6G6D, LY6G6F, NG32 |
| 计算分子量 | 297 aa, 32 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC137212 |
| 基因名称 | LY6G6F |
| Gene ID (NCBI) | 259215 |
| RRID | AB_2880278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SQ64 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LY6G6F, also named as C6orf21, G6F, LY6G6D and NG32, plays a role in the downstream signal transduction pathways involving GRB2 and GRB7. It has monomer (~30 kda) and homodimer (~60 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LY6G6F antibody 25872-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
