验证数据展示
经过测试的应用
| Positive WB detected in | AsPC-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17573 Product name: Recombinant human LY6E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-101 aa of BC119708 Sequence: LMCFSCLNQKSNLYCLKPTICSDQDNYCVTVSASAGIGNLVTFGHSLSKTCSPACPIPEGVNVGVASMGISCCQSFLCNFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte antigen 6 complex, locus E |
| 别名 | 9804, Ly 6E, Ly-6E, Retinoic acid-induced gene E protein, RIGE |
| 计算分子量 | 131 aa, 14 kDa |
| 观测分子量 | 12-16 kDa |
| GenBank蛋白编号 | BC119708 |
| 基因名称 | LY6E |
| Gene ID (NCBI) | 4061 |
| RRID | AB_2879007 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16553 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LY6E antibody 22144-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lab Chip LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier | ||
Carcinogenesis α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer. | ||
J Virol LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.
| ||
PLoS Pathog Glycosylphosphatidylinositol biosynthesis functions as a conserved host defense pathway against coronaviruses via regulation of LY6E. | ||
Breast Cancer Res Nuclear PD-L1 drives IFN-γ-promoted lung metastasis of triple-negative breast cancer via POLR2A-mediated transcriptional activation of LY6E. |
