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验证数据展示

  • FC experiment of human PBMCs using 98626-2-RR

    FC experiment of human PBMCs using 98626-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LTBR Rabbit RecAb (98626-2-RR, Clone:251739F2), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Cells were incubated with FC Receptor Block prior to staining.

  • FC experiment of human PBMCs using 98626-2-RR

    FC experiment of human PBMCs using 98626-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LTBR Rabbit RecAb (98626-2-RR, Clone:251739F2) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue),and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.Cells were incubated with FC Receptor Block prior to staining.

  • FC experiment of HeLa using 98626-2-RR

    FC experiment of HeLa using 98626-2-RR

    1x10^6 HeLa cells were surface stained with 0.25 ug Anti-Human LTBR Rabbit RecAb (98626-2-RR, Clone:251739F2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.

  • Affinity of 98626-2-RR

    Affinity of 98626-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98626-2-RR against Human LTBR were performed. The affinity constant is 2.39 nM.

Anti-Human LTBR Rabbit Recombinant Antibody

LTBR Uni-rAb® Recombinant Antibody for FC
Cat No. 98626-2-RR

产品说明书

CloneNo. 251739F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

D12S370, LT BETA R, Lymphotoxin beta receptor, Lymphotoxin-beta receptor, TNF R III

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs, HeLa cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98626-2-RR targets LTBR in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg5126

Product name: Recombinant Human LTBR protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 31-227 aa of BC026262

Sequence: QAVPPYASENQTCRDQEKEYYEPQHRICCSRCPPGTYVSAKCSRIRDTVCATCAENSYNEHWNYLTICQLCRPCDPVMGLEEIAPCTSKRKTQCRCQPGMFCAAWALECTHCELLSDCPPGTEAELKDEVGKGNNHCVPCKAGHFQNTSSPSARCQPHTRCENQGLVEAAPGTAQSDTTCKNPLEPLPPEMSGTMLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphotoxin beta receptor (TNFR superfamily, member 3)
别名 D12S370, LT BETA R, Lymphotoxin beta receptor, Lymphotoxin-beta receptor, TNF R III
计算分子量 435 aa, 47 kDa
GenBank蛋白编号BC026262
基因名称 LTBR
Gene ID (NCBI) 4055
RRIDAB_3746225
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP36941
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Lymphotoxin-beta receptor (LTBR) is a receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. It can activate the NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA1-LTB2) (PMID: 24248355). LTBR is constitutively expressed on stromal cells and myeloid lineage cells but not on T or B lymphocytes, which may be a novel immune checkpoint of tumor-associated macrophages (PMID: 20603617; 39429877).

实验方案

Product Specific Protocols
FC protocol for LTBR antibody 98626-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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