验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26855-1-AP targets LTBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | LTBP1 fusion protein Ag25392 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | latent transforming growth factor beta binding protein 1 |
| 别名 | LTBP 1, LTBP1 |
| 计算分子量 | 1721 aa, 187 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC130289 |
| 基因名称 | LTBP1 |
| Gene ID (NCBI) | 4052 |
| RRID | AB_2880658 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14766 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein which belongs to the family of latent TGF-beta binding proteins (LTBPs). LTBP1 is essential for TGF-beta folding, secretion, matrix localization and activation. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular matrix components, such as fibrillin. We got 150-240 kDa in western blotting due to protein glycosylation of LTBP1 (PMID: 18086923, PMID: 9923648 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LTBP1 antibody 26855-1-AP | Download protocol |
| IHC protocol for LTBP1 antibody 26855-1-AP | Download protocol |
| IF protocol for LTBP1 antibody 26855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis | ||
J Transl Med LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.
| ||
Cell Death Discov ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency. | ||
medRxiv Secreted protein profiling of human aortic smooth muscle cells identifies vascular disease associations | ||
Adv Mater Engineered Surfaces that Promote Capture of Latent Proteins to Facilitate Integrin-Mediated Mechanical Activation of Growth Factors | ||
Front Immunol Hypoxia reconstructed colorectal tumor microenvironment weakening anti-tumor immunity: construction of a new prognosis predicting model through transcriptome analysis |