验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
21361-1-AP targets LST1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag15817 Product name: Recombinant human LST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-66 aa of BC103856 Sequence: VKRLERSWAQGSSEQELHYASLQRLPVPSSEGPDLRGRDKRGTKEDPRADYACIAENKPT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leukocyte specific transcript 1 |
| 别名 | Protein B144, LST 1, Leukocyte-specific transcript 1 protein, D6S49E, B144 |
| 计算分子量 | 97 aa, 11 kDa |
| 观测分子量 | 10-15 kDa |
| GenBank蛋白编号 | BC103856 |
| 基因名称 | LST1 |
| Gene ID (NCBI) | 7940 |
| RRID | AB_2918071 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00453 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LST1 (Leukocyte Specific Transcript 1), also known as B144, is a small adaptor protein expressed in leukocytes of myeloid lineage. LST1 splice variants (LST1/A-LST1/N) have been detected in various cell types, and thought to affect mainly LST1 gene expression and splicing, are associated with inflammatory conditions(PMID: 10706707, PMID: 33986741). The molecular weight of LSTI protein may vary between different lysates.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LST1 antibody 21361-1-AP | Download protocol |
| IHC protocol for LST1 antibody 21361-1-AP | Download protocol |
| WB protocol for LST1 antibody 21361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
