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验证数据展示

  • WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    WB result of LSR antibody (18216-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LSR transfected HT-29 cells. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LSR Polyclonal antibody, PBS Only

LSR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18216-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ILDR3, Lipolysis-stimulated lipoprotein receptor, LISCH, LISCH7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18216-1-PBS targets LSR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12961

Product name: Recombinant human LSR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 339-589 aa of BC004381

Sequence: SMRVLYYMEKELANFDPSRPGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRRSRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHHRTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPAPPPYSETDSQASRERRLKKNLALSRESLVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipolysis stimulated lipoprotein receptor
别名 ILDR3, Lipolysis-stimulated lipoprotein receptor, LISCH, LISCH7
计算分子量 71 kDa
观测分子量 60-70 kDa, 56 kDa
GenBank蛋白编号BC004381
基因名称 LSR
Gene ID (NCBI) 51599
RRIDAB_2250137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86X29
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).

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