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LSR Polyclonal antibody

LSR Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18216-1-AP

Print datasheet

Synonyms

ILDR3, LISCH, LISCH7, LSR

验证数据展示

  • WB analysis of HT-29 using 18216-1-AP

    WB analysis of HT-29 using 18216-1-AP

    HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 18216-1-AP

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 18216-1-AP

    IHC staining of mouse stomach using 18216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 18216-1-AP

    FC experiment of HepG2 using 18216-1-AP

    1X10^6 HepG2 cells were stained with .2ug LSR antibody (18216-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHT-29 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse stomach tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18216-1-AP targets LSR in WB, FC, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Immunogen LSR fusion protein Ag12961 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipolysis stimulated lipoprotein receptor
Synonyms ILDR3, LISCH, LISCH7, LSR
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa, 56 kDa
GenBank Accession NumberBC004381
Gene Symbol LSR
Gene ID (NCBI) 51599
RRIDAB_2250137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).

实验方案

Product Specific Protocols
WB protocol for LSR antibody 18216-1-APDownload protocol
IHC protocol for LSR antibody 18216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Biosens Bioelectron

A multiple mixed TiO2 mesocrystal junction based PEC-colorimetric immunoassay for specific recognition of lipolysis stimulated lipoprotein receptor.

Authors - Yanjie Chen
View Article
mouseWB

J Hazard Mater

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Authors - Zijun Peng
View Article