验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, PC-3 cells, HEK-293T cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18336-1-AP targets LSM14A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12997 Product name: Recombinant human LSM14A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 100-463 aa of BC016842 Sequence: SSTSSFQSMGSYGPFGRMPTYSQFSPSSLVGQQFGAVGVAGSSLTSFGTETSNSGTLPQSSAVGSAFTQDTRSLKTQLSQGRSSPQLDPLRKSPTMEQAVQTASAHLPAPAAVGRRSPVSTRPLPSASQKAGENQEHRRAEVHKVSRPENEQLRNDNKRQVAPGAPSAPRRGRGGHRGGRGRFGIRRDGPMKFEKDFDFESANAQFNKEEIDREFHNKLKLKEDKLEKQEKPVNGEDKGDSGVDTQNSEGNADEEDPLGPNCYYDKTKSFFDNISCDDNRERRPTWAEERRLNAETFGIPLRPNRGRGGYRGRGGLGFRGGRGRGGGRGGTFTAPRGFRGGFRGGRGGREFADFEYRKDNKVAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LSM14A, SCD6 homolog A (S. cerevisiae) |
| 别名 | AlphaSNBP, C19orf13, FAM61A, hRAP55, hRAP55A |
| 计算分子量 | 463 aa, 51 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC016842 |
| 基因名称 | LSM14A |
| Gene ID (NCBI) | 26065 |
| RRID | AB_10644339 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8ND56 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LSM14A antibody 18336-1-AP | Download protocol |
| IHC protocol for LSM14A antibody 18336-1-AP | Download protocol |
| WB protocol for LSM14A antibody 18336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
Acta Pharmacol Sin NSCLC cells sustain phase separation of cytoplasmic membrane-less organelles to protect themselves against cisplatin treatment | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation | ||
Adv Sci (Weinh) Oleic Acid Dissolves cGAS-DNA Phase Separation to Inhibit Immune Surveillance | ||
Nucleic Acids Res RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules |
