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LRPAP1 Polyclonal antibody

LRPAP1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24662-1-AP

Print datasheet

Synonyms

A2MRAP, A2RAP, Alpha 2 MRAP, HBP44, LRPAP1, MRAP, RAP

验证数据展示

  • WB analysis using 24662-1-AP

    WB analysis using 24662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24662-1-AP (LRPAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 24662-1-AP

    FC experiment of HeLa using 24662-1-AP

    1X10^6 HeLa cells were stained with 0.2ug LRPAP1 antibody (24662-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • IHC staining of human kidney using 24662-1-AP

    IHC staining of human kidney using 24662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24662-1-AP (LRPAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPAP1 antibody included
  • IHC staining of human kidney using 24662-1-AP

    IHC staining of human kidney using 24662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24662-1-AP (LRPAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPAP1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LRPAP1 Ready-To-Use IHC Kit. LRPAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24662-1-AP is LRPAP1 Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen LRPAP1 fusion protein Ag20304 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name low density lipoprotein receptor-related protein associated protein 1
Synonyms A2MRAP, A2RAP, Alpha 2 MRAP, HBP44, LRPAP1, MRAP, RAP
Calculated Molecular Weight 357 aa, 41 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC112067
Gene Symbol LRPAP1
Gene ID (NCBI) 4043
RRIDAB_2879662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRPAP1 antibody 24662-1-APDownload protocol
IHC protocol for LRPAP1 antibody 24662-1-APDownload protocol
FC protocol for LRPAP1 antibody 24662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Res

Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system.

Authors - Jie You
View Article