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验证数据展示

  • IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

    IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LRCH1 antibody included
  • WB analysis of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    WB analysis of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    IP experiment of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    IP result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 25017-1-AP (same clone as 25017-1-PBS)

    IF Staining of MCF-7 using 25017-1-AP (same clone as 25017-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using LRCH1 antibody (25017-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

LRCH1 Polyclonal antibody, PBS Only

LRCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25017-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LRCH1 Ready-To-Use IHC Kit. LRCH1 primary antibody included.

产品信息

25017-1-PBS targets LRCH1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18728

Product name: Recombinant human LRCH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-549 aa of BC112937

Sequence: NIMEEEQIIKEDSCHRLSPVKGEFHQEFQPEPSLLGDSTNSGEERDQFTDRADGLHSEFMNYKARAEDCEELLRIEEDVHWQTEGIISSSKDQDMDIAMIEQLREAVDLLQDPNGLSTDITERSVLNLYPMGSAEALELQDSALNGQIQLETSPVCEVQSDLTLQSNGSQYSPNEIRENSPAVSPTTNSTAPFGLKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeats and calponin homology (CH) domain containing 1
别名 Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1
计算分子量 728 aa, 80 kDa
观测分子量 85-95 kDa
GenBank蛋白编号BC112937
基因名称 LRCH1
Gene ID (NCBI) 23143
RRIDAB_2879848
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2L9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.

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