验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, LNCaP cells |
| Positive IP detected in | LNCaP cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | LNCaP cells |
Planning an IHC experiment? We recommend our IHCeasy LPIN1 Ready-To-Use IHC Kit. LPIN1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
27026-1-AP targets LPIN1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag25815 Product name: Recombinant human LPIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-460 aa of BC030537 Sequence: ICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipin 1 |
| 别名 | EC:3.1.3.4, KIAA0188, lipin 1, Lipin1, Lipin-1 |
| 计算分子量 | 890 aa, 99 kDa |
| 观测分子量 | 110-130 kDa |
| GenBank蛋白编号 | BC030537 |
| 基因名称 | LPIN1 |
| Gene ID (NCBI) | 23175 |
| RRID | AB_2880727 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14693 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| IHC protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| IP protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| WB protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.
| ||
J Agric Food Chem Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism. | ||
Adipocyte Effects of miR-29c on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stromal cells | ||
Toxins (Basel) Combining Multiple Omics with Molecular Dynamics Reveals SCP2-Mediated Cytotoxicity Effects of Aflatoxin B1 in SW480 Cells | ||
Mol Biol Cell Differential reliance of CTD-nuclear envelope phosphatase 1 on its regulatory subunit in ER lipid synthesis and storage | ||
World J Gastroenterol Hepatic-specific vitamin D receptor downregulation alleviates aging-related metabolic dysfunction-associated steatotic liver disease |
