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验证数据展示

  • IHC staining of mouse cerebellum using 20043-1-AP

    IHC staining of mouse cerebellum using 20043-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20043-1-AP (LPHN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 20043-1-AP

    IHC staining of mouse cerebellum using 20043-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20043-1-AP (LPHN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 20043-1-AP

    IF Staining of mouse cerebellum using 20043-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LPHN2 antibody (20043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LPHN2 Polyclonal antibody

LPHN2 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 20043-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

CIRL-2, CIRL2, CIRL 2, Calcium-independent alpha-latrotoxin receptor 2, Adhesion G protein-coupled receptor L2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20043-1-AP targets LPHN2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 latrophilin 2
别名 CIRL-2, CIRL2, CIRL 2, Calcium-independent alpha-latrotoxin receptor 2, Adhesion G protein-coupled receptor L2
计算分子量 163 kDa
GenBank蛋白编号NM_012302
基因名称 LPHN2
Gene ID (NCBI) 23266
RRIDAB_3085596
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPHN2, Latrophilin-2, is a calcium-independent receptor of low affinity for alpha-latrotoxin which triggers massive exocytosis from neurons and neuroendocrine cells. LPHN2 is a novel receptor of LRG1 that rescues vascular and neurological abnormalities (PMID: 35562586). LPHN2 and LPHN3 serve as postsynaptic recognition molecules in CA1 pyramidal neurons of the hippocampus, localized to Purkinje cells in cerebellum (PMID: 32202499).

实验方案

Product Specific Protocols
IHC protocol for LPHN2 antibody 20043-1-APDownload protocol
IF protocol for LPHN2 antibody 20043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols