LPCAT3 Monoclonal antibody
LPCAT3 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig and More (2)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C1G3
验证数据展示
经过测试的应用
| Positive WB detected in | mouse stomach tissue, pig stomach tissue |
| Positive IHC detected in | human stomach tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
产品信息
67882-1-Ig targets LPCAT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, pig |
| Cited Reactivity | human, mouse, rat |
| Immunogen | LPCAT3 fusion protein Ag6472 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lysophosphatidylcholine acyltransferase 3 |
| Synonyms | C3F, LPCAT, LPCAT3, LPLAT 5, LPSAT, Lyso PC acyltransferase, Lyso PC acyltransferase 3, Lyso PS acyltransferase, MBOAT5, nessy, OACT5 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC065194 |
| Gene Symbol | LPCAT3 |
| Gene ID (NCBI) | 10162 |
| RRID | AB_2918639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6P1A2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that play an important role in lipid metabolism and homeostasis by regulating the abundance of different phosphatidylcholine (PC) species. Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is abundantly expressed in the testes, kidneys and metabolic tissues, including liver, intestine and adipose tissues. LPCAT3 is involved in the occurrence and development of atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH)(PMID: 35711841).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LPCAT3 antibody 67882-1-Ig | Download protocol |
| IHC protocol for LPCAT3 antibody 67882-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Toxicol Di-(2-ethylhexyl) phthalate induces ferroptosis in prepubertal mouse testes via the lipid metabolism pathway | ||
J Exp Clin Cancer Res Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progression. | ||
Ecotoxicol Environ Saf Cadmium perturbed lipid profile and induced liver dysfunction in mice through phosphatidylcholine remodeling and promoting arachidonic acid synthesis and metabolism | ||
Sci Total Environ Insight into the effect of a heavy metal mixture on neurological damage in rats through combined serum metabolomic and brain proteomic analyses | ||
Redox Biol Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis | ||
J Immunother Cancer Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors
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