• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LPAR4 Polyclonal antibody

LPAR4 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 22165-1-AP

Print datasheet

Synonyms

G protein coupled receptor 23, GPR23, LPA 4, LPA receptor 4, LPA4, LPAR4, P2RY9, P2Y purinoceptor 9, P2Y5 LIKE, P2Y5 like receptor, P2Y9, Purinergic receptor 9

验证数据展示

  • WB analysis using 22165-1-AP

    WB analysis using 22165-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 22165-1-AP

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 22165-1-AP

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of HL-60 using 22165-1-AP

    FC experiment of HL-60 using 22165-1-AP

    1X10^6 HL-60 cells were stained with 0.2ug LPAR4 antibody (22165-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inSKOV-3 cells, mouse brain tissue, HL-60 cells, 3T3-L1 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 22165-1-AP is LPAR4 Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LPAR4 fusion protein Ag17817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysophosphatidic acid receptor 4
Synonyms G protein coupled receptor 23, GPR23, LPA 4, LPA receptor 4, LPA4, LPAR4, P2RY9, P2Y purinoceptor 9, P2Y5 LIKE, P2Y5 like receptor, P2Y9, Purinergic receptor 9
Calculated Molecular Weight 370 aa, 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC069996
Gene Symbol LPAR4
Gene ID (NCBI) 2846
RRIDAB_11182491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPAR4 antibody 22165-1-APDownload protocol
IHC protocol for LPAR4 antibody 22165-1-APDownload protocol
FC protocol for LPAR4 antibody 22165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation

Authors - Chengsheng Wu
View Article