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CoraLite® Plus 488-conjugated LOXL2 Monoclonal antibody

LOXL2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7F1

Cat No : CL488-67139

Print datasheet

Synonyms

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

验证数据展示

  • WB analysis using CL488-67139

    WB analysis using CL488-67139

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67139 (LOXL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy LOXL2 Ready-To-Use IHC Kit. LOXL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67139-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67139 targets LOXL2 in WB applications and shows reactivity with Human samples.

Tested Applications WB
Tested Reactivity Human
Immunogen LOXL2 fusion protein Ag28566 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name lysyl oxidase-like 2
Synonyms LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14
Calculated Molecular Weight 774 aa, 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000594
Gene Symbol LOXL2
Gene ID (NCBI) 4017
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4K0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 LOXL2 antibody CL488-67139Download protocol
Standard Protocols
Click here to view our Standard Protocols