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验证数据展示

  • WB analysis using 15232-1-AP

    WB analysis using 15232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15232-1-AP

    WB analysis using 15232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15232-1-AP

    IHC staining of human breast cancer using 15232-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LOXL2 antibody included
  • IHC staining of human breast cancer using 15232-1-AP

    IHC staining of human breast cancer using 15232-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LOXL2 antibody included
  • IF Staining of HepG2 using 15232-1-AP

    IF Staining of HepG2 using 15232-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 15232-1-AP

    IF Staining of HepG2 using 15232-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LOXL2 Polyclonal antibody

LOXL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15232-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

EC:1.4.3.13, LOR2, Lysyl oxidase-related protein WS9-14, WS9 14, WS914

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LOXL2 Ready-To-Use IHC Kit. LOXL2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15232-1-AP targets LOXL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag7083

Product name: Recombinant human LOXL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 428-774 aa of BC000594

Sequence: AMGLQKKLRLNGGRNPYEGRVEVLVERNGSLVWGMVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGDVNSNKVVMSGVKCSGTELSLAHCRHDGEDVACPQGGVQYGAGVACSETAPDLVLNAEMVQQTTYLEDRPMFLLQCAMEENCLSASAAQTDPTTGYRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTHYDLLNLNGTKVAEGHKASFCLEDTECEGDIQKNYECANFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESDYSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysyl oxidase-like 2
别名 EC:1.4.3.13, LOR2, Lysyl oxidase-related protein WS9-14, WS9 14, WS914
计算分子量 774 aa, 87 kDa
观测分子量68 kDa. 100 kDa
GenBank蛋白编号BC000594
基因名称 LOXL2
Gene ID (NCBI) 4017
RRIDAB_3085455
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4K0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.

实验方案

Product Specific Protocols
IF protocol for LOXL2 antibody 15232-1-APDownload protocol
IHC protocol for LOXL2 antibody 15232-1-APDownload protocol
WB protocol for LOXL2 antibody 15232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Authors - Xiaochao Tan
View Article
mouseWB

J Ethnopharmacol

Escin Ia Ameliorates DSS-Induced Chronic Colitis in Mice by Inhibiting Inflammation and Oxidative Stress via the LOXL2/MMP-9 Pathway

Authors - Jing Yan
View Article
humanWB

J Cancer

Dihydroartemisinin Potentiates VEGFR-TKIs Antitumorigenic Effect on Osteosarcoma by Regulating Loxl2/VEGFA Expression and Lipid Metabolism Pathway

Authors - Xiaomin Ding
View Article
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