验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, mouse lung tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24300-1-AP targets LIPI in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | LIPI fusion protein Ag19024 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | lipase, member I |
别名 | Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5 |
计算分子量 | 460 aa, 55 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC148691 |
基因名称 | LIPI |
Gene ID (NCBI) | 149998 |
RRID | AB_2879483 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6XZB0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for LIPI antibody 24300-1-AP | Download protocol |
IHC protocol for LIPI antibody 24300-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |