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验证数据展示

  • WB analysis of A549 using 24300-1-AP

    WB analysis of A549 using 24300-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24300-1-AP

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 24300-1-AP

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 40x lens).

LIPI Polyclonal antibody

LIPI Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24300-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24300-1-AP targets LIPI in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19024

Product name: Recombinant human LIPI protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 264-481 aa of BC148691

Sequence: FSGIQFIKCNHQRAVHLFMASLETNCNFISFPCRSYKDYKTSLCVDCDCFKEKSCPRLGYQAKLFKGVLKERMEGRPLRTTVFLDTSGTYPFCTYYFVLSIIVPDKTMMDGSFSFKLLNQLGMIEEPRLYEKNKPFYKLQEVKILAQFYNDFVNISSIGLTYFQSSNLQCSTCTYKIQRLMLKSLTYPERPPLCRYNIVLKDREEVFLNPNTCTPKNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipase, member I
别名 Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5
计算分子量 460 aa, 55 kDa
观测分子量 50 kDa
GenBank蛋白编号BC148691
基因名称 LIPI
Gene ID (NCBI) 149998
RRIDAB_2879483
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6XZB0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for LIPI antibody 24300-1-APDownload protocol
WB protocol for LIPI antibody 24300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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