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LIPG Monoclonal antibody

LIPG Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B10B10

Cat No : 67434-1-Ig

Print datasheet

Synonyms

EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719

验证数据展示

  • WB analysis using 67434-1-Ig

    WB analysis using 67434-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human prostate cancer using 67434-1-Ig

    IHC staining of human prostate cancer using 67434-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCaco-2 cells, HaCaT cells, HepG2 cells, HT-29 cells, human placenta tissue, MCF-7 cells, MDA-MB-468 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67434-1-Ig targets LIPG in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen LIPG fusion protein Ag29482 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lipase, endothelial
Synonyms EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 and 42 kDa
GenBank Accession NumberBC060825
Gene Symbol LIPG
Gene ID (NCBI) 9388
RRIDAB_2882672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y5X9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIPG antibody 67434-1-IgDownload protocol
IHC protocol for LIPG antibody 67434-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols