验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, HaCaT cells, HepG2 cells, HT-29 cells, human placenta tissue, MCF-7 cells, MDA-MB-468 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67434-1-Ig targets LIPG in WB, IHC, CoIP, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, CoIP |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | LIPG fusion protein Ag29482 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | lipase, endothelial |
别名 | EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719 |
计算分子量 | 57 kDa |
观测分子量 | 57 and 42 kDa |
GenBank蛋白编号 | BC060825 |
基因名称 | LIPG |
Gene ID (NCBI) | 9388 |
RRID | AB_2882672 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9Y5X9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for LIPG antibody 67434-1-Ig | Download protocol |
IHC protocol for LIPG antibody 67434-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |