验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells, HuH-7 cells, rat liver tissue, mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87243-1-RR targets LIPC in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15538 Product name: Recombinant human LIPC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-463 aa of BC146659 Sequence: DASFVDAIHTFTREHMGLSVGIKQPIGHYDFYPNGGSFQPGCHFLELYRHIAQHGFNAITQTIKCSHERSVHLFIDSLLHAGTQSMAYPCGDMNSFSQGLCLSCKKGRCNTLGYHVRQEPRSKSKRLFLVTRAQSPFKVYHYQLKIQFINQTETPIQTTFTMSLLGTKEKMQKIPITLGKGIASNKTYSFLITLDVDIGELIMIKFKWENSAVWANVWDTVQTIIPWSTGPRHSGLVLKTIRVKAGETQQR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lipase, hepatic |
| 别名 | EC:3.1.1.3, EC:3.1.1.32, EC:3.1.1.5, HL, HTGL |
| 计算分子量 | 499 aa, 56 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC146659 |
| 基因名称 | LIPC |
| Gene ID (NCBI) | 3990 |
| RRID | AB_3745425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11150 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIPC, also named as HTGL and HL, belongs to the AB hydrolase superfamily and Lipase family. It has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. LIPC is an important enzyme in HDL metabolism. It binds heparin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIPC antibody 87243-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
