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验证数据展示

  • IF Staining of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    IF Staining of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    FC experiment of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LIMK2 Recombinant antibody (82977-1-RR, Clone:230283D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82977-1-RR

    Affinity and Kinetic Characterization of 82977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

LIMK2 Recombinant monoclonal antibody, PBS Only

LIMK2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82977-1-PBS

产品说明书

CloneNo. 230283D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LIMK2 Ready-To-Use IHC Kit. LIMK2 primary antibody included.

产品信息

82977-1-PBS targets LIMK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3011

Product name: Recombinant human LIMK2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 379-686 aa of BC013051

Sequence: KLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LIM domain kinase 2
别名 LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10
计算分子量 686 aa, 78 kDa
GenBank蛋白编号BC013051
基因名称 LIMK2
Gene ID (NCBI) 3985
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53671
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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