LIMK1 Polyclonal antibody

LIMK1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 19699-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA and More (1)

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LIMK1 Ready-To-Use IHC Kit. LIMK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19699-1-AP targets LIMK1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用IHC, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain kinase 1
别名 LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1
计算分子量 73 kDa
GenBank蛋白编号NM_002314
基因名称 LIMK1
Gene ID (NCBI) 3984
RRIDAB_10696174
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53667
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.

实验方案

Product Specific Protocols
IHC protocol for LIMK1 antibody 19699-1-APDownload protocol
IF protocol for LIMK1 antibody 19699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang
mouse

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu
humanIHC

Oncol Rep

FOXD3‑AS1/miR‑128‑3p/LIMK1 axis regulates cervical cancer progression.

Authors - Xiufang Yang
human

Cancer Med

MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.

Authors - Nengquan Sheng
human

Cell Death Discov

FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.

Authors - Xinyan Jiao
human

Mol Carcinog

LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling

Authors - Guanghui Hu
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