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EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66071-1-Ig

产品说明书

CloneNo. 6E6E6

宿主/亚型

Mouse / IgG1

种属反应性

human, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, rat
文献引用反应性human, mouse
免疫原 EPLIN fusion protein Ag13288 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM domain and actin binding 1
别名 LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624
计算分子量 85 kDa
观测分子量 90 kDa, 110 kDa
GenBank蛋白编号BC001247
基因名称 EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UHB6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

实验方案

Product Specific Protocols
WB protocol for EPLIN antibody 66071-1-IgDownload protocol
IHC protocol for EPLIN antibody 66071-1-IgDownload protocol
IF protocol for EPLIN antibody 66071-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Adv Sci (Weinh)

LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease

Authors - Fuji Yang
humanWB,CoIP

Cell Biol Toxicol

M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway

Authors - Chao Zhang
mouse,humanWB,IHC,IF,CoIP

Cell Mol Biol Lett

Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy

Authors - Shihui Li
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