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验证数据展示

  • WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPLIN antibody included
  • IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPLIN Monoclonal antibody, PBS Only

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66071-1-PBS

产品说明书

CloneNo. 6E6E6

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® EPLIN Ready-To-Use IHC Kit. EPLIN primary antibody included.

产品信息

66071-1-PBS targets EPLIN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag13288

Product name: Recombinant human LIMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 261-401 aa of BC001247

Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM domain and actin binding 1
别名 LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624
计算分子量 85 kDa
观测分子量 90 kDa, 110 kDa
GenBank蛋白编号BC001247
基因名称 EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHB6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

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